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A) TUNEL assay: Positive apoptosis cells is brilliant florescent green (TUNEL+) and negative apoptosis cells is pale and opaque green (TUNEL-). B) Acridine Orange test: positive AO cells are yellow (AO+) and negative cells with normal DNA exhibited in green color (AO-). C) Sperm chromatin dispersion (SCD) test: Big halo represents no DNA fragmentation (SCD-) and no halo and small halo show sperm with DNA fragmentation (SCD+) (×1000 magnification
