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Expression validation of miRNAs through qRT-PCR. The differential expression of miRNAs was validated using 17 miRNAs in 22 different comparisons. The fold change expression of miRNAs was calculated from NGS data and qPCR experiment to compare their expression. The y-axis shows the fold change expression of miRNAs. Bars represent the mean±SE of three biological replicates. (This figure is available in color at JXB online.)
