Figure 1.

Characterization of the cdt2^TR allele. (A) cdt2^TR cells were grown in minimal sporulation liquid (MSL) medium at 30 °C. The culture was divided in two, thiamine was added to the indicated culture, and pictures were taken with Nomarski optics after 12 h; (B) cdt2^TR cells of the indicated genotype were grown at 30 °C in MSL medium. At t = 0, thiamine was added to the four cultures. Samples were passed through flow cytometry at hourly intervals, as indicated. The apparent slight drift to the left at early time points in the Δspd2 strain was due to a DNA staining artefact; (C) Serial dilutions of strains with the indicated genotypes were spotted on plates either with or without thiamine, and incubated at the indicated temperature for three days; (D) cdt2^TR cells expressing VN173-pcn1 and spd1-VC155 [6] were propagated in minimal sporulation liquid (MSL). The culture was divided in two, thiamine was added to the indicated culture, and pictures of yellow fluorescent protein (YFP) fluorescence were taken after four hours; (E) DNA content profiles of growing wild type and Δcdt2 cells.