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Spd1 accumulation inhibits S phase progression. Cells with the cdt2TR allele were synchronized in G1 by nitrogen starvation in the presence of the M-factor pheromone [22]. The pheromone was washed away, and the cultures were released into S phase in MSL medium at 30 °C, either with or without thiamine at t = 0. Samples were taken for flow cytometry at the indicated time points (min). Genotypes: (A) cdt2TR; (B) cdt2TR Δspd1; (C) cdt2TR Δspd2 and (D) cdt2TR Δspd1 Δspd2.
