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. 2017 May 6;8(5):134. doi: 10.3390/genes8050134

Figure 2.

Figure 2

Identification of a novel conserved domain essential for MYC-mediated transactivation. (A) Sequence comparison of the N-terminal 45 amino acids of MYC from several different species and MYC family members. Stars identify exact amino acid matches and periods denote chemically similar amino acids. MYC Box 0 (MB0) is defined by the box containing amino acids 10–32 of human MYC. The regions of polyglutamine (Poly Q) amino acids are illustrated by boldface; (B) Schematic showing specific deletions of MB0, PolyQ region and MBI in Gal4MYC100 used in the Gal4 assays; (C) The MB0 domain is essential for MYC-mediated transactivation. Gal4 assays were performed and analyzed as described in Figure 1 using the various Gal4MYC100 deletion constructs described above.