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. 2017 May 6;8(5):134. doi: 10.3390/genes8050134

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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MB0 is essential for induction of MYC target genes. Stable p53^−/−/ARF^−/− DKO MEFs expressing MYCER, MYCER∆MB0, MYCER∆MBI or empty vector were treated with 2 µM hydroxytamoxifen (OHT) for 0 or 12 h. Quantitative Real-Time RT-PCR (qRT-PCR) was performed as described in the Materials and Methods used to assess the levels of target genes Rcl, Hsp60 and Cdk4. Results were normalized to ß-actin levels and shown as fold induction over levels at 0 h. Open bars indicate mRNA levels prior to OHT treatment, closed bars indicate mRNA levels after 12 h OHT treatment. Asterisks indicate statistically significant increases in mRNA after treatment with OHT as determined by Student’s t-test (p < 0.05, n = 3 per treatment group). MYCER protein expression of the various constructs was determined by IB analysis.

MB0 is essential for induction of MYC target genes. Stable p53^−/−/ARF^−/− DKO MEFs expressing MYCER, MYCER∆MB0, MYCER∆MBI or empty vector were treated with 2 µM hydroxytamoxifen (OHT) for 0 or 12 h. Quantitative Real-Time RT-PCR (qRT-PCR) was performed as described in the Materials and Methods used to assess the levels of target genes Rcl, Hsp60 and Cdk4. Results were normalized to ß-actin levels and shown as fold induction over levels at 0 h. Open bars indicate mRNA levels prior to OHT treatment, closed bars indicate mRNA levels after 12 h OHT treatment. Asterisks indicate statistically significant increases in mRNA after treatment with OHT as determined by Student’s t-test (p < 0.05, n = 3 per treatment group). MYCER protein expression of the various constructs was determined by IB analysis.