Figure 6.

MB0, but not MBI, is essential for efficient MYC-induced p53-indendent apoptosis in MEFs. Stable p53^−/− MEFs expressing MYCER, MYCER∆MB0 or MYCER∆MBI were shifted to low serum and treated with 2 µM OHT or ethanol as described in the Materials and Methods. The percentage of dead cells was determined daily. All cultures treated with OHT showed statistically significant increases in cell death by Day 2 of treatment as determined by Student’s t-test (p < 0.05). MYCER protein expression of the various constructs was determined by IB analysis.