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LPS reduces ATP production in HGF-1 cells. To determine ATP production following LPS treatment ((a), 5 μg/mL for 48 h), Magnesium Green fluorescence (503/530 nM) was determined during respiration after calibration with ADP (2.5 mM) (n = 3). The rate of peak ATP production was then compared against the rate of oxygen consumption to determine the P : O ratio ((b), n = 3). ∗P < 0.05.
