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. 2017 May 30;7:46126. doi: 10.1038/srep46126

Figure 2. TG693 promotes skipping of mutated exon 31 in HeLa cells in a dose-dependent manner.

Figure 2

(a) SR protein phosphorylation was assessed in HeLa cells treated with TG693 and TG003 for 1 h. Lamin B served as a loading control. Uncropped images have been provided in Supplementary Fig. S4. (b,c) Effect of TG693 on exon 31 skipping with the reporter plasmid. Transfected HeLa cells were incubated in the presence of TG693, TG003, or DMSO vehicle for 24 h. Reporter and endogenous CLK1 splicing was then analyzed by RT-PCR. GAPDH served as a control. The Splicing ratios were quantified by intensity analysis and normalized to GAPDH expression. Uncropped images have been provided in Supplementary Figs S5 and S6, respectively. Data represent the means ± SD (n = 3).