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. 2017 Apr 3;292(21):8630–8641. doi: 10.1074/jbc.M116.770719

Figure 2.

Figure 2.

Curing of different [PSI+] variants was measured upon overexpression of different Hsp104 fragments. A–C, curing of different [PSI+] variants was measured in yeast overexpressing Sc-Hsp104ΔC7 or Sc-Hsp104ΔC24 from the GAL1 promoter. The dashed lines are the curing curves from Fig. 1A obtained by overexpressing full-length Sc-Hsp104. D, Sc-Hsp104ΔC52 was overexpressed in different [PSI+] variants. Curing was measured at the indicated times using the red/white colony assay. E, 5-fluoroorotic acid (5-FOA) shuffle experiment using 1408 yeast in which the pJ312 plasmid, which encodes Sc-Hsp104 under the control of the S. cerevisiae HSP104 promoter on a URA3-based centromeric plasmid, was shuffled with empty vector or plasmids expressing full-length Sc-Hsp104 or the indicated C-terminal truncations of Sc-Hsp104. The full-length and fragments of Sc-Hsp104 were under the control of the Sc-HSP104 promoter. Only constructs that support [PSI+] propagation grow on plates without adenine.