Figure 12.

Predictive analysis for the presence of CPPs within the N- and C-terminal sequences of the MF6p/HDM family of proteins. The presence of CPP regions was predicted with the CPPpred bioinformatics tool as indicated under “Experimental procedures.” In addition to the N- and C-terminal sequences derived from the trematode MF6p/HDM family of proteins, three well known CPPs were analyzed as controls with the same tool. The score indicates the probability (p range, 0–1) of the peptides being CPPs. Scores of peptides being CPPs with a probability of ≥50% are shaded in green. The positive CPP control sequences derived from the proteins Antennapedia (Penetratin; residues 43–58), human immunodeficiency virus type 1 Tat (residues 48–60), and murine vascular endothelium cadherin (pVEC; residues 615–632), respectively (25).