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Comparison of hemin spectra in several solvent systems. A, UV/visible absorption spectra of hemin (12 μm) in TBS (pH 7.3), TBS-T, or TBS-C. B, UV/visible absorption spectra of hemin (12 μm) alone, mixed with LSZ, or with PMX at protein to hemin molar ratios of 2:1 and 1:1 in TBS-C. Addition of LSZ or PMX did not shift the absorbance maxima of hemin spectra. AU, absorbance units.
