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. 2017 Mar 31;292(21):8716–8728. doi: 10.1074/jbc.M116.767574

Figure 5.

Figure 5.

Regulation of HNF4α protein stability by hypoxia or metformin. A, MIN6 cells were incubated at 5% O2 or 20% O2 for 18 h, and HNF4α protein stability was examined for another 6 h using CHX (10 μg/ml). Relative HNF4α protein levels are indicated as percentage values (n = 3–4). Half-lives of HNF4α protein are indicated. B, MIN6 cells were incubated with or without metformin (2 mm) for 18 h, and HNF4α protein stability was examined. Relative HNF4α protein levels are indicated as percentage values (n = 3–4). Half-lives of HNF4α protein are shown. C, experimental scheme using the MG132 proteasome inhibitor. D, effect of MG132 (10 μm) on HNF4α expression by hypoxia or metformin treatment. E and F, relative HNF4α protein levels in 5% O2 (hypoxia) (E) or metformin (F) were calculated (n = 3). All data are presented as mean ± S.E. (error bars). N.S., not significant; *, p < 0.05; **, p < 0.01.