Table 1.

Binding assay data for ERK2-IQGAP1 interaction

Experimenta	Bindingb	Kdc
	%	μm
A-1	37.5	3.0
A-2	25.1	5.3
A-3	18.8	7.7
A-4	16.3	9.2
A-5	16.7	8.9
A-6	28.9	4.4
A-7	11.9	13.2
A-8	16.4	9.1
A-9	19.2	7.5
Mean	21.2	7.6
Median	18.8	7.7
Standard deviation	7.9	3.1
Standard error	2.6	1.0

^a Binding reactions (200 μl) contained ∼1 pmol (∼5 nM) ³⁵S-labeled, in vitro translated, full-length IQGAP1 and 25 μg (1.8 μm) of GST-hERK2 fusion protein. Every experiment contained duplicate points (also known as technical replicates); these were averaged to obtain the “% binding” number shown.

^b Percent of the input ³⁵S-labeled protein that bound to the GST fusion protein.

^c Calculated based on the known input concentrations and percent binding, as described elsewhere (65).