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. 2017 Mar 29;292(21):8823–8834. doi: 10.1074/jbc.M117.780957

Figure 6.

Figure 6.

Grp78 knockdown induces fat body cell ER stress and apoptosis. A, RNAi efficiency of Grp78-1 and Grp78-2 in the fat body of 2–6-day-old adult females treated with dsRNA shared by both Grp78-1 and Grp78-2 (iGrp78). iGFP, dsGFP control; *, p < 0.5; **, p < 0.01; ***, p < 0.001 (n = 6–8). B, the relative expression of Grp94, PDI, and Grp170 in the fat body of Grp78-depleted (iGrp78) adult females at 2–6 days PAE. iGFP, dsGFP control; *, p < 0.05, **, p < 0.01; n.s., no significant difference (n = 6–8). C, relative expression of Grp78-1, Grp78-2, Ire1, Grp94, PDI, and Grp170 in the fat body of adult females in three groups at 6 days PAE. iGFP, dsGFP control; iGrp78, dsGrp78-injected group; iGrp78+iIre1, dsGrp78- and dsIre1-injected group. *, p < 0.5; **, p < 0.01; ***, p < 0.001; n.s., no significant difference (n = 8). D, the relative mRNA levels of Ire1, Grp78-1, Grp78-2, Grp94, PDI, Grp170, and VgA in iGFP versus iIre1 groups. **, p < 0.01; n.s., no significant difference (n = 8). E, apoptotic cell detection in Grp78-depleted (iGrp78) fat body (Fb) cells. H33342, Hoechst 33342 for staining of nuclei (blue); TUNEL, detection of apoptotic cells (green), highlighted by yellow arrows. Scale bar, 200 μm.