Table 1.
Primers used for qRT-PCR or RNAi
| Gene | Forward primer | Reverse primer | |
|---|---|---|---|
| qRT-PCR | Met | CCACTTACAGGCTTGCTA | GCCCTTCTTCACCTTCTT |
| Mcm4 | GGCGAATTGCGGAACCTACC | GCAGGCATATCATCAGGCGATT | |
| Mcm7 | ACGAGTTTGACAAGATGGCTGAC | ACGACCATAGGCTGGATTTGC | |
| Grp78-1 | GGGGACACTCATTTGGGTG | TTGCTTTTTCTACTTCACGGC | |
| Grp78-2 | TGTTAGTAGGTGGAAGCACAAGGA | GCACAGCAGCACCATATACAACT | |
| Ire1 | TTCTTCTGGGCGAGCACTAAGT | ACAGCAACCAGGCCACTCTC | |
| Grp94 | CACTTCTGCCCAGGATCTCAAT | GCTTGTCATCATTGTGCTTGGT | |
| PDI | GACAACTCTCCACGGCGGAA | GGTAGACTGTACGGAAGCTGGT | |
| Grp170 | AGACCTTGACCATCTTCCTGAACA | GTGCGTGTCCTTGTGCTTGG | |
| VgA | CCCACAAGAAGCACAGAACG | TTGGTCGCCATCAACAGAAG | |
| Rp49 | CGTAAACCGAAGGGAATTGA | GAAGAAACTGCATGGGCAAT | |
| DmMet | CGTCCTTAGATTCGCCACCC | GAGGCAGACATACCCGTTCC | |
| DmGce | CTCAGTCCCTTCACCTTCAT | ACCTTGTTCGTCTCCTTGTC | |
| DmTai | AGCGATGTAAAGCCCGAGA | AAAGCAGCATTCCACCCAC | |
| Dmβ-actin | ACTTCTGCTGGAAGGTGGAC | ATCCGCAAGGATCTGTATGC | |
| RNAi | Grp78-1 | TATAACCTGCGAACGAGTA | CACTAGGAATGCAATACAGA |
| Grp78-2 | GTTCTAGTGAGGATGATGAC | TTCAGATATGTAGAGCTAAG | |
| Grp78 | AAGGACAACCATCTGCTAGGAA | GGCTGAACCACATCTTCTAACTC | |
| Met | TTAGGGCAGCATCAGAAAG | TCGTCGGGAGGAAGTGTAT | |
| Mcm4 | CGCCGTGAAAGAGGTGAAGAAG | AAGCAGGTGGAACTGACTGATGA | |
| Mcm7 | GGAACAGCAGACCATTACCATT | ACATCAGCCAGCCTCAGAC | |
| Ire1 | CGAAGATCACCAACACCGATT | GTCCTTCCAATGAGCACAGATT | |
| GFP | CACAAGTTCAGCGTGTCCG | GTTCACCTTGATGCCGTTC | |
| DmMet | CTGCCAACTATCCGATTGTCTC | CTCTCGCCGTAGTCACTGTT | |
| DmTai | AGCATCAGCACCAGCATCA | GTCGTTGTCGTAGAGTTGTTGT |