Table 1.
Primers used in qPCR experiments
| Gene (PubMed accession number) | Primer sequence | Cell-type specificity | Primer efficiency (%) |
|---|---|---|---|
| Atp1b2 (NM_013415.5) | |||
| Forward | TGTGTTGGCAAGAGAGATGAAGA | Astrocytesa | 99.8 |
| Reverse | GGGTCACATTCAGGAACTTTACA | ||
| Slc1a3 (NM_148938.3) | |||
| Forward | CCTGGGTTTTCATTGGAGGGTTG | Astrocytes | 104.2 |
| Reverse | GTGGCAGAACTTGAGGAGGTC | ||
| Aldh1l1 (NM_027406.1) | |||
| Forward | GCAGGTACTTCTGGGTTGCT | Astrocytes | 90.67 |
| Reverse | GGAAGGCACCCAAGGTCAAA | ||
| Cx3cr1 (NM_009987) | |||
| Forward | CGTGAGACTGGGTGAGTGAC | Microglia | 93.20 |
| Reverse | GGACATGGTGAGGTCCTGAG | ||
| Mbp (NM_001025251.2) | |||
| Forward | CATCCTTGACTCCATCGGGC | Oligodendrocytes | 93.75 |
| Reverse | CAGGGTACCTTGCCAGAGC | ||
| Ocln (NM_008756.2) | |||
| Forward | CCGCCAAGGTTCGCTTATCT | Endothelia | 97.67 |
| Reverse | CGGACATGGCTGATGTCACT | ||
| Cspg4 (NM_139001.2) | |||
| Forward | AGGCTGAGGTAAATGCTGGG | Oligodendrocyte precursors | 97.67 |
| Reverse | GCAGGTGGTGAGGACAGTAG | ||
| Dcx (NM_001110222.1) | |||
| Forward | AGGTAACGACCAAGACGCAAA | Neuronal precursors | 97.00 |
| Reverse | GGGTGTAGAGATAGGAGACTGC | ||
| Syt1 (NM_001252341.1) | |||
| Forward | GGGGAAGGGAAGGAAGATGC | Neurons | 99.67 |
| Reverse | ACGGTGGCAATGGGATTTTATG | ||
| Tbp (NM_013684.3) | |||
| Forward | GCCAAGAGTGAAGAACAATCCA | Housekeeping gene | 94.33 |
| Reverse | AGAACTTAGCTGGGAAGCCC | ||
| Cyc1 (NM_025567.2) | |||
| Forward | TGCTCTTCCTGCCACAGC | Housekeeping gene | 93.67 |
| Reverse | GACCTCCACCTCCTCAGCC | ||
a As based on expression data in this paper.