Table 2.
Antibodies used in flow cytometry experiments
All antibodies used in flow cytometry were used according to the manufacturer's instructions (including recommended dilutions), except for anti-O1-eFluor 660, which was used at a 1:80 dilution.
| Antibody-fluorophore conjugate | Vendor and product code | Cell-type specificity |
|---|---|---|
| ACSA-2-PE | Miltenyi Biotec: 130102365 | Astrocytes |
| ACSA-2-APC | Miltenyi Biotec: 130102315 | Astrocytes |
| Anti-GLAST-PE | Miltenyi Biotec: 130095821 | Astrocytes |
| Anti-GLAST-APC | Miltenyi Biotec: 130098804 | Astrocytes |
| Anti-CD11B-PE | Miltenyi Biotec: 130098087 | Microglia |
| Anti-CD31-PE | Miltenyi Biotec: 130102971 | Endothelia |
| Anti-THY1.2-PE | Miltenyi Biotec: 130102960 | Neuronsa |
| Anti-NG2-PE | Miltenyi Biotec: 130097458 | Oligodendrocyte precursors |
| Anti-O4-APC | Miltenyi Biotec: 130095891 | Oligodendrocytes |
| Anti-O1-eFluor 660 | eBioscience: 506506 | Oligodendrocytes |
a Note the anti-THY1.2-PE antibody has also been reported to partially stain glia (28).