Table 4.

shRNAs used for ATP1B2 knockdown experiments

shRNA constructs specific for mouse ATP1B2 were purchased from Origene. In addition to encoding shRNAs, these constructs also contained cytosolic GFP to act as a marker for cell transfection. Constructs were amplified using a Midiprep kit (Macherey-Nagel) and verified by sequencing. Constructs were then transfected into cells using the Neon electroporation system. A scrambled shRNA sequence was used as a negative control.

shRNA target	shRNA identifier	shRNA sequence
ATP1B2	TL500159A	CATGTTCAGAAGCTCAACAAGTTCTTGGA
ATP1B2	TL500159B	CCAAGATGGTCATCCAGAAAGAGAAGAAG
ATP1B2	TL500159C	CCAAGACTGAGAACCTTGATGTCATTGTC
ATP1B2	TL500159D	CGTGTGGCCTTCAAACTCCGGATCAACAA
Scrambled shRNA	TR30021	GCACTACCAGAGCTAACTCAGATAGTACT