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. 2017 Mar 30;292(21):8907–8917. doi: 10.1074/jbc.M116.762898

Figure 1.

Figure 1.

A, RCR is the ratio of state 3 respiration (ADP stimulated) to state 4 respiration (no ADP present), and is considered an indication of the degree of coupling of mitochondrial respiratory chain activity to oxidative phosphorylation. RCR of mitochondria in permeabilized SHY5Y control and VCP knockdown cells (KD#1, KD#2, KD#3) in the presence of vehicle control, uncoupling protein inhibitors (GDP; Genipin), or ANT inhibitors (CATR; BKA). B, SHY5Y SCR and KD cells were probed for VCP, ANT1, UCP2, and β-actin by Western blotting (cropped). C, quantification of VBasal/Voligomycin in permeabilized SHY5Y control and VCP knockdown cells as an alternative indicator of coupling of mitochondrial respiratory chain activity to oxidative phosphorylation. D, full-length representative Western blot analysis of whole cell lysates from stable SCR SH-SY5Y cells and stable VCP knockdown SH-SY5Y cells probing for mitochondrial complexes and β-actin. *, p < 0.05; **, p < 0.01; ***, p < 0.001.