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. 2017 Mar 24;292(21):8933–8947. doi: 10.1074/jbc.M116.770420

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Figure 2. — Lentivirus-mediated ARRB knockdown in U251 and U87 cells. Cells were transfected by lentivirus shRNA against ARRB1, ARRB2, or sh-c. The protein and mRNA levels of the target protein were detected by Western blot and QPCR. A and B, the protein and mRNA levels of ARRB1in U251, U251-Sh-c, U251-ARRB1-sh1, and U251-ARRB1-sh2. C and D, the protein and mRNA levels of ARRB2 in U251, U251-Sh-c, U251-ARRB2-sh1, and U251-ARRB2-sh2. D and E, the protein and mRNA levels of ARRB1 in U87, U87-Sh-c, U87-ARRB1-sh1, and U87-ARRB1-sh2. Total Akt served as a control. The QPCR data were normalized to β-actin levels. The results are means ± S.E. for three independent experiments in triplicate. ***, p < 0.001 versus scramble shRNA.