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. 2017 Mar 24;292(21):8933–8947. doi: 10.1074/jbc.M116.770420

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Figure 4. — ARRB1 is required for NK1R-mediated U251 and U87 cell proliferation. hHK-1 was added to cells transfected with shRNA against ARRB1 (Sh-1 or Sh-2) or sh-c. A, dose-dependent cell proliferation induced by hHK-1 was measured by colony formation assay (the top panel is representative of colony images from the indicated cell type, and the bottom panel shows quantitative analysis of the colony formation assay). B and C, CellTiter-Glo assay (B) and growth curve (C) of the indicated types of cells treated with 1 μm hHK-1 (determined by CellTiter-Glo assay). The results are means ± S.E. for three independent experiments performed in triplicate. ctl, control.