Figure 3.

CRY affects BRP degradation in the distal lamina. BRP levels in the distal lamina were measured as immunofluorescence intensity on cryosections of the optic lobe at ZTs 1, 4, 13 and 16. (A,D) In Canton S flies the immune-signal was bimodal with one peak at the beginning of the day (ZT1) and one at the beginning of the night (ZT13). (B,E) In cry⁰¹ mutants, lack of CRY resulted in high BRP immunofluorescence across the whole day (ZTs 1, 4, 13) but a decrease in the middle of the night (ZT16). (C,F) In cry > CRY∆ overexpressing flies the BRP immune-signal was lower at every time point compared to the other two genotypes. However, a peak was observed at ZT13. (G,H) In the parental lines cry-Gal4 and UAS-CRY∆ the BRP immune-signal was similar to the one from Canton S, with two peaks at ZT1 and ZT13. Immunofluorescence intensities are reported as Mean ± SD. N = 30, three repetitions. Statistical significance was calculated by ANOVA using Tukey’s HSD test. The letters (a,b,c) above the bars represent statistical differences (p < 0.05). Namely, the same letter labeling two or more bars indicates that there are not statistical differences between the values. Conversely, two different letters indicate a significant difference between the labeled bars.