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. 2017 May 9;114(21):5497–5502. doi: 10.1073/pnas.1619336114

Fig. S1.

Fig. S1.

Erdr1 is up-regulated in germfree T cells and regulated by microbial signals. (A) Heat map of the most significantly changed genes in germfree mice compared with SPF in splenic T cells in the KEGG category of cellular maintenance. n = 2/group. (B) Ingenuity Analysis on microarray data from A showing the most significantly different pathways when comparing splenic germfree (GF) versus SPF CD4+ T cells. (C) CD3+CD4+ T-cell lysate from GF or SPF spleens was collected and analyzed for Erdr1 protein levels. (D) Total bacteria from fecal samples of animals after 2 wk of antibiotic treatment. (E) BALB/c mice were treated with antibiotics for 2 wk. CD3+CD4+ T cells were isolated from the spleen, and Erdr1 was measured by qRT-PCR. n = 4–5/group. (F) CD3+CD4+ T cells were isolated from the indicated compartments, and levels of Erdr1 were measured by qRT-PCR. n = 3/group. (G) RNA was collected from naive (CD3+CD4+CD44−CD62L+), activated (CD3+CD4+CD44+CD62L−), or Treg (CD3+CD4+CD25hi) T cells, and levels of Erdr1 were analyzed. (H) CD3+CD4+ T cells from GF spleens were collected and incubated with either Pam3 or Imiquimod, and Erdr1 was measured by qRT-PCR. n = 3/group. *P < 0.05; **P < 0.01. Statistical significance was determined by Student’s t test.