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. 2017 May 8;114(21):E4251–E4260. doi: 10.1073/pnas.1618310114

Fig. S1.

Fig. S1.

MHV nsp15 mutant viruses induce rapid cell death in macrophages. (A) Clustal W was used to align the complete amino acid sequence of nsp15 from the listed CoVs, with the region surrounding the highly conserved T98 and H262 depicted. (B) Growth kinetics in 17Cl-1 cells (MOI 0.1). Values were analyzed using a nonlinear regression test. **P < 0.01; ***P < 0.001. (C) ifnar−/− BMDMs were infected at an MOI of 0.1 and harvested at indicated times for CellTiter Glo assay; n.s., not significant. (D and E) Cytopathic effect was observed under bright-field microscopy (magnification, 100×), with rapid cell death in nsp15 mutant viruses infected B6 BMDM (D) and syncytia formation in all virus-infected ifnar−/− BMDMs (E). Data are representative of two to three independent experiments and error bars represent the mean ± SD.