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. 2017 May 9;114(21):5539–5544. doi: 10.1073/pnas.1619820114

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Fig. S4. — Effects of dsRNA acr-16+acr-26 on motility and quantitative PCR demonstrates no effect on motility following soaking for 5 d but a reduction in acr-16 and acr-26 mRNA. The effect of dsRNA acr-16+acr-26 contrasts with the effect on motility of dsRNA unc-38+unc-29. (A) Time series of motility for dsRNA soaked worms. Black (circles) representing control, blue (squares) lacZ (nonspecific dsRNA), red (triangles) (dsRNA unc-29 + unc-38), and green (triangles) (dsRNA acr-16+acr-26) color data points representing the different treatments of worms (n = 10, four biological replicates for each treatment, two-way ANOVA and Bonferroni post hoc tests; ***P < 0.001). (B) Bar chart demonstrating quantitative PCR results showing a significant reduction in transcript levels of acr-16 (hatched green, by 93 ± 2.0%) and acr-26 (filled green by 92 ± 1.1%) compared with the control transcript levels in lacZ dsRNA-treated worms, acr-16 (hatched blue by 27 ± 2.4%) and acr-27 (filled blue by 17 ± 4.9%). n = 6 worms. (unpaired t tests: ***P < 0.001; **P < 0.01; *P < 0.05). ds, double stranded.