Fig. 4.

Kinetics measurements of α_vβ₈ and α_vβ₆ binding to pro-TGF-β1 by SPR. (A) Sensorgrams show the overlay of fitting curve (thicker gray) and experimental curve (thinner black) of SPR measurement. Constructs and metal ions used are indicated. Concentrations were 800, 400, 200, 100, 50, 25, and 0 nM for the α_vβ₈ ectodomain; 400, 200, 100, 50, 25, 10, and 0 nM in Mg²⁺ and 200, 100, 50, 25, 10, and 0 nM in Mn²⁺ for the α_vβ₈ headpiece; and 200, 100, 50, 20, and 0 nM in Mg²⁺ and 100, 50, 20, 10, 5, and 0 nM in Mn²⁺ for the α_vβ₆ ectodomain. (B) Affinities and kinetic rates. Values are mean ± difference from mean of two independent experiments.