. 2017 May 8;114(21):E4193–E4202. doi: 10.1073/pnas.1704016114

Table 1.

Restoration of the WT Hsp104 (at normal levels) cures most of the spontaneously arising [PSI⁺] variants

Donor HSP104 genotype and [PSI⁺] variant (phenotype on 1/2 YPD)	Recipient	Cytoductants	Ade⁺ cytoductants	% Ade⁺	P value
WT [PSI⁺1] (ss)	WT	12	11	92
WT [PSI⁺1] (ss)	hsp104^T160M	9	9	100
WT [PSI⁺2] (ss)	WT	12	2	17	6 × 10⁻⁵
WT [PSI⁺2] (ss)	hsp104^T160M	16	16	100	6 × 10⁻⁵
WT [PSI⁺3] (ss)	WT	13	12	92
WT [PSI⁺3] (ss)	hsp104^T160M	14	14	100
hsp104^T160M [PSI⁺4] (vwu)	WT	32	13	41	4 × 10⁻⁴
hsp104^T160M [PSI⁺4] (vwu)	hsp104^T160M	22	21	95	4 × 10⁻⁴
hsp104^T160M [PSI⁺5] (vwvu)	WT	13	0	0
hsp104^T160M [PSI⁺5] (vwvu)	hsp104^T160M	12	0	0
hsp104^T160M [PSI⁺6] (vwu)	WT	21	17	81
hsp104^T160M [PSI⁺6] (vwu)	hsp104^T160M	15	15	100
hsp104^T160M [PSI⁺7] (vwu)	WT	23	6	26	1 × 10⁻⁴
hsp104^T160M [PSI⁺7] (vwu)	hsp104^T160M	15	12	80	1 × 10⁻⁴
hsp104^T160M [PSI⁺8] (vwu)	WT	3	0	0	2 × 10⁻³
hsp104^T160M [PSI⁺8] (vwu)	hsp104^T160M	19	19	100	2 × 10⁻³
hsp104^T160M [PSI⁺9] (vwvu)	WT	37	6	16	1 × 10⁻⁵
hsp104^T160M [PSI⁺9] (vwvu)	hsp104^T160M	18	15	83	1 × 10⁻⁵
hsp104^T160M [PSI⁺10] (vwu)	WT	28	18	64	1 × 10⁻⁵
hsp104^T160M [PSI⁺10] (vwu)	hsp104^T160M	21	20	95	1 × 10⁻⁵
hsp104^T160M [PSI⁺11] (vwvu)	WT	26	7	27	1 × 10⁻⁵
hsp104^T160M [PSI⁺11] (vwvu)	hsp104^T160M	14	14	100	1 × 10⁻⁵

The spontaneously arising prion variants isolated in either WT (AG666) or hsp104^T160M (AG667) background were used as cytoduction donors to isogenic WT (AG686) or hsp104^T160M (AG687) recipients. The cytoduction efficiency of the [PSI⁺2], [PSI⁺4], [PSI⁺7], [PSI⁺8], [PSI⁺9], [PSI⁺10], and [PSI⁺11] prion variants into hsp104^T160M recipients was significantly higher than into WT recipients. ss, strong stable; vwu, very weak unstable; vwvu, very weak very unstable (Fig. S1B). Statistical tests were carried out as described in Methods.