Table S1.
Sequences for Gel Shift and Filter-Binding Assays
| Item | Name | Sequence |
| 1 | sgRNA | GACGCAUAAAGAUGAGACGCGUUUUAGAGCUAUGCUGUUUUGGAAACAAAACAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUUUUGCUCGUGCGCUGGAUC |
| 2 | sgRNA_probe_Cy3 | /5Cy3/AAGATCCAGCGCACGAGCAA |
| 3 | Flow_cell_probe_Alexa647 | /5Alex647N/TCTACACATCGATGTGCATTGAACGAT |
| 4 | Cas9_WT_array_construct | CAAGCAGAAGACGGCATACGAGAT AAGGGGCGAGTTGAT TGAAATGCTTGCGACTTACGAGTGCTATGCGACTGCACAGCAGAAATCTCTGCT CCAGCGTCTCATCTTTATGCGTC AGTACAAACGTCAGCATCGTTCAATGCACATCGATGTGTAGATCTCGGTGGTCGCCGTATCATT |
| 5 | Cas9_−16G_array_construct | CAAGCAGAAGACGGCATACGAGAT TGAAGGCGTAATATT TGAAATGCTTGCGACTTACGAGTGCTATGCGACTGCACAGCAGAAATCTCTGCT CCAGCGTCTCATCTTTATCCGTC AGTACAAACGTCAGCATCGTTCAATGCACATCGATGTGTAGATCTCGGTGGTCGCCGTATCATT |
| 6 | Cas9_−16T_array_construct | CAAGCAGAAGACGGCATACGAGAT GGGCATTCAACTCCG TGAAATGCTTGCGACTTACGAGTGCTATGCGACTGCACAGCAGAAATCTCTGCT CCAGCGTCTCATCTTTATACGTC AGTACAAACGTCAGCATCGTTCAATGCACATCGATGTGTAGATCTCGGTGGTCGCCGTATCATT |
| 7 | Cas9_−13C_array_construct | CAAGCAGAAGACGGCATACGAGAT TCGTTTGGAAAACCT TGAAATGCTTGCGACTTACGAGTGCTATGCGACTGCACAGCAGAAATCTCTGCT CCAGCGTCTCATCTTGATGCGTC AGTACAAACGTCAGCATCGTTCAATGCACATCGATGTGTAGATCTCGGTGGTCGCCGTATCATT |
| 8 | Cas9_−5T_array_construct | CAAGCAGAAGACGGCATACGAGAT GGCGCCGCAGCATCA TGAAATGCTTGCGACTTACGAGTGCTATGCGACTGCACAGCAGAAATCTCTGCT CCAGCGTATCATCTTTATGCGTC AGTACAAACGTCAGCATCGTTCAATGCACATCGATGTGTAGATCTCGGTGGTCGCCGTATCATT |
| 9 | Cas9_+3A_array_construct | CAAGCAGAAGACGGCATACGAGAT ATCAGCTTTGGCGTA TGAAATGCTTGCGACTTACGAGTGCTATGCGACTGCACAGCAGAAATCTCTGCT TCAGCGTCTCATCTTTATGCGTC AGTACAAACGTCAGCATCGTTCAATGCACATCG ATGTGTAGATCTCGGTGGTCGCCGTATCATT |
| 10 | C_adapter | AATGATACGGCGACCACCGAGATCTACAC |
| 11 | D_adapter | CAAGCAGAAGACGGCATACGAGAT |
| 12 | D_adapter_Cy5 | /5Cy5/CAAGCAGAAGACGGCATACGAGAT |