Figure 3.

Experimental time series of total monomeric protein (GlnB, GlnK, and GS) and metabolite (glutamine and α-KG) concentrations for the WT, ΔglnB, and ΔglnK strains. Symbols and error bars represent average protein or metabolite concentrations mean ± SE (n = 3). The gray-shaded area marks the starvation period. The lines are guides to the eye. This data was used to drive the model (pictured) of the PTM of the three proteins (highlighted). Time-course measurements of the modified proteins GS-A, GlnB-U, and GlnK-U were also taken experimentally and are presented in Fig. 5.