Figure 5.

Time responses of PTM protein levels in the WT and two knock-out mutants. (a) Time responses of GlnB uridylylation, GlnK uridylylation, and GS adenylylation in the WT strain (experimental measurements and fitted model, with and without sequestration). (b and c) Experimental measurements of GlnB uridylylation, GlnK uridylylation, and GS adenylylation for knock-outs: (b) ΔglnB and (c) ΔglnK, compared in both cases to the dynamics predicted by our model fitted only to the WT data. The symbols and error bars represent measured average protein concentrations mean ± SE (n = 3). The lines correspond to fits and prediction of our model, as indicated. The gray-shaded area marks the starvation period. The model with sequestration provides a significantly better fit according to information criteria for model selection (see text).