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. 2004 Dec 15;3:50. doi: 10.1186/1475-2875-3-50

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Copyright © 2004 Flick et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Impact of bacterial growth stages on protein solubility. The expression of the three recombinant proteins was induced at various timepoints chosen gradually from low OD A₆₀₀to higher OD A₆₀₀. The comparison of the soluble and insoluble fractions of the recombinant proteins revealed that after initiation of expression at an OD A₆₀₀of greater than 2.0, the recombinant proteins are found almost completely in the soluble fraction. The amount of each protein loaded is not proportional to the size of the cultivation.