TABLE 3.

ANALYSIS OF PERIPHERAL BLOOD CELL SUBSETS

	Before Randomization (SAC 1)		After Randomization (SAC 2)
Cell Type %	Placebo	Abatacept	Placebo	Abatacept	P Value
CD4+ T cell	47.3 ± 6.6	44.2 ± 7.3	45.5 ± 15.1	45.3 ± 10.7
Naive	48.8 ± 14.2	37.0 ± 10.3	49.7 ± 14.5	44.2 ± 10.4	0.041
Memory	46.2 ± 13.5	54.7 ± 10.3	45.2 ± 14.1	47.3 ± 11.0	0.013
CD28+CD25+	14.7 ± 8.5	13.9 ± 4.2	15.3 ± 6.1	15.4 ± 5.0
CD28+CD25−	83.9 ± 8.6	83.5 ± 5.3	83.3 ± 6.9	81.6 ± 7.9
CD8+ T cell	26.0 ± 3.7	32.0 ± 6.1	23.7 ± 8.5	28.1 ± 6.1
Naive	71.9 ± 11.4	69.1 ± 12.5	71.8 ± 8.9	69.6 ± 11.9
Memory	21.6 ± 9.5	22.5 ± 9.0	22.0 ± 8.0	22.9 ± 9.8
CD28+CD25+	0.7 ± 0.8	0.7 ± 0.6	0.7 ± 0.7	1.1 ± 0.6	0.08
CD28+CD25−	57.5 ± 17.0	53.0 ± 13.5	61.3 ± 14.2	55.3 ± 9.4
B cell	17.6 ± 5.1	15.8 ± 6.0	15.7 ± 5.5	17.4 ± 6.8
Nk cell	15.7 ± 12.2	19.9 ± 13.3	18.7 ± 16.4	16.1 ± 9.5
Treg	3.2 ± 3.1	3.7 ± 1.5	3.9 ± 2.2	3.1 ± 1.9
MDSC (Lin1−)	17.8 ± 16.7	28.3 ± 20.3	13.5 ± 14.2	13.3 ± 16.3
Myeloid DC (Lin1−)	2.4 ± 2.1	3.0 ± 3.4	3.1 ± 2.8	1.9 ± 1.2
Plasmacytoid DC (Lin1−)	1.4 ± 1.2	1.3 ± 1.0	1.3 ± 1.3	1.1 ± 0.9

Definition of abbreviations: DC = dendritic cell; MDSC = myeloid-derived suppressor cell; SAC = segmental allergen challenge.

Peripheral blood was collected before bronchoscopy at the prerandomization allergen challenge time point and again before the second SAC at the end of the study intervention period (after randomization) and analyzed by flow cytometry for specific cell subsets. Shown are the mean ± standard deviation of each subset as a percentage of the lymphocyte gate. DC and MDSC are shown as a percentage of the lineage cocktail negative gate. P values were calculated comparing whether the postrandomization values were different between placebo and abatacept after correcting for differences present at the prerandomization time point.