Figure 2.

Effect of the dopamine uptake inhibitor GBR 1209 on dopamine release in hypoxic cells. If PC-12 cells were incubated in the presence of 5 nM of the dopamine uptake inhibitor GBR 1209, no effect was observed in cells incubated under normoxic conditions or in a xenon atmosphere. However, a much higher extracellular dopamine concentration was found in cells incubated in N₂ and GBR 1209 than in N₂ alone, indicating that indeed the release was intensified under hypoxic conditions. (n = 4; P < 0.0001 with respect to untreated controls, analyzed by 2-way ANOVA and Bonferroni posttests between GBR1209-treated and untreated groups).