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Sirt1 mRNA (A) and protein (B) expressions in differentiated 3T3L1 cells. (A) Sirt1 mRNA expression was measured by real-time PCR. The data are expressed as mean ± SD of three experiments. *P < 0.05, **P < 0.01 vs 0 μM. (B) Sirt1 protein expression was measured by Western blot. The result shown is representative of three experiments.
