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. 2017 Apr 24;108(5):1071–1079. doi: 10.1111/cas.13213

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© 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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(a) Fluorescence in situ hybridization (FISH) analysis using a spectrum red labeled BACH2 probe and spectrum green labeled centromeric probe for chromosome 6 (CEP6). Epstein–Barr virus (EBV)‐positive diffuse large B‐cell lymphoma (DLBCL) yielded a signal ratio of 90.5%. (b–d) BACH2 expression in human non‐Hodgkin's lymphoma cell lines. (b) BACH2 expression was not detected by PCR in the FL‐18‐EB cell line. (c) BACH2 was expressed in FL‐18 cells but not in FL‐18‐EB cells. (d) FL‐18 and FL‐18‐EB showed signal ratios of 94% and 93%, respectively, by FISH analysis with the BACH2 probe.