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Effects of estradiol (E2) on the mRNA expression levels of GLI1 (a) and GLI2 (b) in four estrogen receptor (ER)‐positive breast cancer cell lines. All the cell lines were treated with the estrogen‐deprived medium plus minus 1 nM E2 for 3 days. The expression levels were measured as described in the Materials and Methods. The values were analyzed after normalization to the controls and expressed as the means ± SE. *P < 0.05; **P < 0.01 in comparison with the control.
