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. 2017 May 5;108(5):838–845. doi: 10.1111/cas.13193

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© 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Antigen‐specific immune responses after personalized peptide vaccination (PPV). T cell and IgG responses specific to the vaccine peptides were determined by interferon‐γ (IFN‐γ) ELISPOT and bead‐based multiplexed assays before and after (6^th and 12^th vaccination) PPV, respectively. (a) The relative changes of peptide‐specific IFN‐γ spot numbers after PPV compared to those before PPV were estimated. The spot numbers before PPV were set to 1.0. Means and standard errors are shown. (b) The relative changes of peptide‐specific IgG titers after PPV compared to those before PPV were estimated. The IgG titers before PPV were set to 1.0. Means and standard errors are shown.