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Independent variables and their levels
| Independent variable | Symbol | Level
|
||
|---|---|---|---|---|
| −1 | 0 | +1 | ||
| Phospholipid concentration (mM) | X1 | 10 | 40 | 70 |
| Curcumin concentration (mM) | X2 | 1 | 5 | 9 |
| Doxorubicin concentration (mM) | X3 | 0.25 | 0.5 | 0.75 |
| Working temperature (°C) | X4 | 45 | 55 | |
| Buffer pH (units) | X5 | 4.5 | 5 | |
| Phospholipid:cholesterol molar ratio | X6 | 5 | 10 | 15 |
