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Four different techniques used for spheroid cultures. (a) A well of low-adhesion plates that have a round bottom with an ultralow cell attachment coating.12 (b) A droplet of hanging drop plate where cells are partitioned and self-organized into a spheroid.13 (c) Suspension culture in bioreactor where cells become self-aggregated into spheroids.14 (d) A representative pillar of micropatterned plates where the cells are enriched on the top of the pillar to form a spheroid.15
