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. 2017 Mar 16;5(2):75–83. doi: 10.1016/j.prnil.2017.03.002

Fig. 6.

Fig. 6

Quantitative and semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis. Bar graphs showing the quantitative RT-PCR expression levels of interleukin-8 (IL-8) and cyclooxygenase-2 (COX-2). (A) Prostate tissue samples. (B) Prostate cancer cell line (PC3). (C) Bar graph showing fold change in cyclin D1 and A2 expression. Bar graphs showing the comparison in expression levels of IL-8 and COX-2. (D) Prostate tissue samples. (E) Prostate cancer cell line (PC3). (F) Ratio of cyclin B1 normalized with β-actin was carried out by semiquantitative RT-PCR. *P < 0.05, **P < 0.01 versus control. NP, normal prostate; ns, not significant; PC, prostate cancer; WS, Withania somnifera.