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. 2017 May 30;12(5):e0178152. doi: 10.1371/journal.pone.0178152

Fig 5. In vitro chronic prostate inflammation model in ihPSC induced by interferon-γ (IFN-γ) and IL-17.

Fig 5

(A) Schematic illustration of in vitro chronic prostate inflammation model and anti-inflammatory effects of hylan G-F 20. (B) Cell proliferation, (C) RT-PCR analysis of prostate inflammation-associated markers and their relative quantification, GAPDH was used as internal control. (D) the expression levels of iNOS and p65 proteins (β-actin and lamin B used as respective internal control) in ihPSC cells treated with IFN-γ and IL-17 (20 ng/ml each) for 48h in the absence or presence of 250, and 500 μg/ml of hylan G-F 20 compared to the control (CTRL) group. Results are shown as the mean±SD for three independent experimental cultures. * and ** indicates a significant difference with P<0.01 and P<0.001, respectively.