Table 1. Primer sets for PCR amplification.
Genes | Primer sequences (5’→3’) |
---|---|
HPV-16 E6/E7 |
ATG CAT AGT ATA TAG AGA TGG GAA T CTG CAG GAT CAG CCA TGG TAG A |
Vimentin |
CTA CAT CGA CAA GGT GCG CTT TGC CAG AGA CGC ATT GTC AA |
α-SMA |
CCA GCT ATG TGA AGA AGA AGA GG GTG ATC TCC TTC TGC ATT CGG T |
human basic (h1) Calponin |
GAG TGT GCA GAC GGA ACT TCA GCC GTC TGT GCC CAA CTT GGG GTC |
Androgen receptor |
CAT GCA CAA GTC CCG GAT G GGT GAG CTG GTA GAA GCG T |
Interlukin1β |
AAA GCT TGG TGA TGT CTG GT TCT ACA CTC TCC AGC TGT AG |
IL-6 |
GGT ACA TCC TCG ACG GCA TCT GTG CCT CTT TGC TGC TTT CAC |
IL-8 |
ATG ACT TCC AAG CTG GCC GTG GCT TCT CAG CCC TCT TCA AAA ACT TCT C |
COX2 |
TTC AAA TGA GAT TGT GGG AAA ATT GCT AGA TCA TCT CTG CCT GAG TAT CTT |
iNOS |
GAT GAG AGT GGC AGC TAC TGG GTC TCC GCA CAA AGC AGG GCA CTG GGT C |
TLR4 |
TGG ATA CGT TTC CTT ATA AG GAA ATG GAG GCA CCC CTT C |
GAPDH |
GCT CTC CAG AAC ATC ATC CCT GC CGT TGT CAT ACC AGG AAA TGA GC |