Table 2. Characteristics of ISSR and SSR markers.
Primer (pairs) | Sequence (5'-3') | Repeat motif | Ta(°C) | Size(bp) | GenBank Accession no. | |
---|---|---|---|---|---|---|
808 | AGAGAGAGAGAGAGAGC | - | - | - | - | |
813 | CTCTCTCTCTCTCTCTT | - | - | - | - | |
834 | AGAGAGAGAGAGAGAGYT | - | - | - | - | |
840 | GAGAGAGAGAGAGAGAYT | - | - | - | - | |
847 | CACACACACACACACARC | - | - | - | - | |
849 | GTGTGTGTGTGTGTGTYA | - | - | - | - | |
ISSR | 892 | TAGATCTGATATCTGAATTCCC | - | - | - | - |
BML4 | f: AGGGACAAGGCGAGCAAG | (TG)7 | 58 | 175–190 | KU161120 | |
r: CAGCATCGGAGTACACAGCA | ||||||
BML8 | f: ACGTACGAGTGCTTTTCCG | (AC)10 | 56 | 209–230 | KU161121 | |
r: GAAGCCTCGCTAATAAAGTCAG | ||||||
BML9 | f: AGCTCTATCGGCTGTTGGGT | (AG)21 | 59 | 141–176 | KU161122 | |
r: TGATTGGCTTCTCATTGGGAC | ||||||
BML11 | f: ACAAATCTGTGAGACTGTGACCG | (AG)21 | 59 | 141–178 | KU161123 | |
r: GGTGCGAATCATGAGGACAG | ||||||
BML12 | f: ACTTTGCTTAAAGGAGTTCATCAAG | (AG)12 | 59 | 157–184 | KU161124 | |
r: AGGGTGGCAAAAATGGGCT | ||||||
BML16 | f: TGATTGATTGGCTCCCCTC | (GA)9 | 58 | 138–165 | KU161125 | |
r: AGGCAGAGTTTCTCCAGGCT | ||||||
BML19 | f: AGGCTATAACCACCGGAACA | (CT)12T(TC)5 | 57 | 187–223 | KU161126 | |
r: ATTGCACGGTCCAGAGAGAC | ||||||
BML21 | f: CTCTGCTGAACCAGCGAAGA | (TC)9 | 59 | 117–135 | KU161127 | |
r: ATTTGGGTCACAAATACACTTGC | ||||||
BML23 | f: CACACACTCACGCGCACAG | (TC)25(AC)7 | 59 | 87–133 | KU161113 | |
r: ACCTGCCAAGGTACATCACG | ||||||
BML27 | f: ACGTCACCTTCAGCAGCG | (AG)13 | 58 | 203–230 | KU161114 | |
r: AGTCCTCATTTCCTCAACGTCT | ||||||
BML30 | f: ATGACGGGGCAAGCAGAG | (GA)16 | 59 | 141–167 | KU161115 | |
r: ACGGTTACTATGGGACCACATCT | ||||||
BML35 | f: AACGGCGCGACAAAACT | (GA)9(GA)6 | 57 | 122–143 | KU161116 | |
r: AGGCCCACCTCTTTTAATTCT | ||||||
BML39 | f: AAATACACTTGCCCATTGGC | (AG)10 | 58 | 113–129 | KU161117 | |
r: ATCTGCTCTGCTGAACCAGC | ||||||
BML45 | f: AGACAATATCGGTCGTTACGC | (AG)16 | 58 | 156–190 | KU161118 | |
r: AGTGACCACTGCCCAGTGTG | ||||||
BML49 | f: AGCTCTTCTTTCCGCGTTTC | (GA)17 | 59 | 118–149 | KU161119 | |
SSR | r: CATGTGGCGCAACTCCTC |