Table IV.

Second-level tests.

Extensive red blood cell typing in anticipation of possible transfusion

Further immune-haematological investigations: C3, C4, CH50

Auto-antibodies (ANA, anti DNA), antiphospholipid antibodies, RA test - Thyroid function and anti-thyroid antibodies (anti-TG, anti-TPO) - Lymphocyte subpopulations (CD3, CD4, CD8, CD19, CD16, CD56) - Double-negative T cells: CD3+, CD4−, CD8−, TCR alpha/beta+

Hepatitis B and C, markers and HIV serology

Coagulation screen blood test

Serum total protein and protein electrophoresis

Immunoglobulin class quantification

C-reactive protein

EBV, CMV, Parvovirus B19, HSV serology

Other assessments for infectious diseases, as clinically appropriate

C3: complement component 3; C4: complement component 4; CH50: total component activity; ANA: antinuclear antibodies; RA test: rheumatoid factor test; TG: thyroglobulin antibody; TPO: thyroid peroxidase antibody; HIV: human immunodeficiency virus; EBV: Epstein-Barr virus; CMV: cytomegalovirus; HSV: herpes simplex virus.