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rF6Hb expression as detected by immunoblotting using anti-His antibody (A) Time course induction of rF6Hb expression. Soluble fraction represents total cell lysate. (B) Purification of rF6Hb on Ni-NTA; Lanes: 1, flow-through (10 μg); 2, wash (5 μg); 3, Bound 1 (1 mL eluate, 2 μg); 4, Bound 2 (2 mL eluate, 2 μg). (C) Purification of rF6Hb eluted from Superose 12 (10 μg/fraction).
