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. 2004 Dec 9;24(1):209–220. doi: 10.1038/sj.emboj.7600441

Figure 1.

Figure 1

Cdk5, p35, p25 and CIP expression in coinfected primary cortical neurons. E18 rat embryonic cortical neurons were infected with the following expression constructs: empty LV-GFP vector (EV), p25 and wild-type Cdk5 (Cdk5WT) with or without CIP, p35 and wild-type Cdk5 with or without CIP, p25 and dominant-negative Cdk5 (Cdk5DN) and p35 and Cdk5DN. (A) After 3 days of infection, neuronal lysates were resolved on 4–20% SDS–PAGE gradient gels and Western blotting was employed to detect endogenous and transfected Cdk5 (C-8; top panel), p35 and p25 (C-19; bottom panel). Lane 1: EV only; lanes 2 and 3: p25+Cdk5WT with and without CIP respectively; lanes 4 and 5: p35+Cdk5WT with and without CIP respectively; lane 6: p25+Cdk5DN; lane 7: p35+Cdk5DN. (B) Confocal images illustrate the cortical neurons single infections with Cdk5, p35, p25 and CIP (panels a–d, respectively), double infection of Cdk5 and p25 (panels e–h), double infection of Cdk5 and p35 (panels j–m), triple infections of Cdk5, p25 and CIP (panels n–r) and Cdk5, p35 and CIP (panels s–w). The scale bar represents 20 μm.