Skip to main content
. 2004 Dec 2;24(1):160–169. doi: 10.1038/sj.emboj.7600486

Figure 1.

Figure 1

Endogenous Mdm2 is associated with endogenous pRB. Exponentially growing HCT116 cells were treated with 20 μM MG115, a proteasome inhibitor, for 8 h. The cell lysates were subjected to IP with anti-Mdm2 (SMP-14) (A) or anti-pRB (G3-245) (B) and control immunoglobulin. The immunoprecipitates were separated by SDS–6% PAGE and then subjected to IB with anti-Mdm2 (SMP-14) or anti-pRB (G3-245) for detection of endogenous pRB and Mdm2. In all, 1% of the total lysate for IP was loaded in an input lane.