Skip to main content
. 2004 Dec 2;24(1):160–169. doi: 10.1038/sj.emboj.7600486

Figure 5.

Figure 5

Accumulation of pRB in Mdm2−/− MEFs. Lysates prepared from p53−/− or p53−/−Mdm2−/− (A), Skp2+/+, Skp2+/− or Skp2−/− (B) or wild-type (WT) or ARF−/− (C) MEFs were subjected to IB with antibodies against p130, p107, Mdm2 and α-tubulin. pRB was immunoprecipitated with anti-pRB antibody and analyzed with the antibody. (D) MEFs were treated with or without 20 μM MG115 for 10 h. pRB was immunoprecipitated with anti-pRB antibody and analyzed by immunoblotting with the antibody. α-Tubulin levels in 6% of the total material used are shown in input lanes. (E) MEFs were treated with 10 μg/ml of cycloheximide for the indicated time, and the endogenous pRB levels were analyzed as described in Materials and methods.