Figure 8.

Inverse correlation between pRB and Mdm2 expressions in human lung cancers. (A) Immunohistochemical staining for pRB and Mdm2 in human non-small cell lung cancer tissues was carried out using streptavidin–biotin–peroxidase complex methods as described in Materials and methods. Original magnification is × 400. (B) The graph shows the levels of pRB expression of non-small cell lung carcinomas (n=30). pRB expressions were classified according to Mdm2 expression (low, moderate or high). Eight of the 30 tissue samples showed signs of Mdm2 overexpression, and Mdm2 expression was low in eight samples. The other 14 samples showed a moderate expression level of Mdm2. pRB expression levels were significantly decreased in Mdm2-high samples. ^*P<0.05, ^**P<0.005.